Composite

Part:BBa_K4759201:Design

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)


T7-RBS1-camA-RBS2-camB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1366
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1366
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1366
    Illegal BamHI site found at 1360
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1366
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1366
    Illegal AgeI site found at 124
    Illegal AgeI site found at 418
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

codon-optimized


Source

camA and camB are from Pseudomonas putida

References